The prevalence of cholelithiasis among 20 to 60 years was unknown. We aimed to access the prevalence among this corresponding age group. As cholelithiasis is a gall stone disease and it is one of the common gall bladder diseases. In this disease, gall stones are formed which are hyperechoic in structure and they are three types like cholesterol stones, pigment stones, and mixed stones. Numerous diagnostic methods are used for the diagnosis of cholelithiasis are ultrasound scan, CT imaging, ERCP or MRCP, and HIDA tests. Among all of them, and Ultrasound scan was considered best in this study because of its specificity which was 99%. It was observed that Gall stone disease had an association with the patient age family history and gender.